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Aspergillopepsin II
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Aspergillopepsin II : ウィキペディア英語版
Aspergillopepsin II

Aspergilloglutamic peptidase, also called aspergillopepsin II (, ''proctase A'', '' Aspergillus niger acid proteinase A'', ''Aspergillus niger var. macrosporus aspartic proteinase'') is a proteolytic enzyme. The enzyme was previously thought be an aspartic protease, but it was later shown to be an glutamic protease with a catalytic Glu residue at the active site, and was therefore renamed aspergilloglutamic peptidase.
Determination of its molecular structure showed it to be a unique two-chain enzyme with a light chain and a heavy chain bound non-covalently with each other. The C-terminal region of the light chain of one molecule binds to the active site cleft of another molecule in the manner of a substrate.
This enzyme catalyses the following chemical reaction
: Preferential cleavage in B chain of insulin: Asn3-Gln, Gly13-Ala, Tyr26-Thr
This enzyme is isolated from ''Aspergillus niger var. macrosporus''.
== References ==


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